What is two-photon microscopy

Two-photon microscopy, also known as two-photon excitation (2PE) microscopy, is a fluorescence imaging technique that allows for the observation of living tissues up to a depth of about one millimeter. In the two-photon excitation model, a molecule simultaneously absorbs two photons whose individual energy is only half of the energy needed to excite that molecule, and then releases the energy to a fluorescence photon. Two-photon microscopy (TPM) generally uses a near infrared excitation wavelength laser that reduces the tissue autofluorescence and optical scattering. It offers a powerful tool for observing the intricate processes within living organisms, especially for studying the dynamics of neurons, neural circuits, and other cellular interactions in deep tissues.

What is miniature two-photon microscopy

An important goal of neuroscientists is to establish a connection between changes in the structure and function of neuronal circuits and changes in behavior. It is therefore crucial to study these phenomena in intact, behaving animals. By miniaturizing two-photon excitation (2PE) microscopes into devices small and light enough to be mounted on the heads of small animals, such as mice, it becomes possible to perform real-time, in vivo imaging of neuronal and cellular activities in the brain of an animal engaging in natural behaviors.
What’s so special about miniature two-photon microscopy

What’s so special about miniature two-photon microscopy

Sub-cellular resolution
Single-spike resolution
1000+ neurons
Cell type-specific
Free-behaving animal imaging
Longitudinal study of the same population of cells
Applications in neuroscience

Applications in neuroscience

Imaging subjects: Neurons, dendritic spines, microglia, astrocytes, neurovasculature, immune cells, mitochondria, etc.
Research subjects: Brain, cerebellum, brainstem, spinal cord, liver, pancreatic islets
Neural circuit studies: Pain, itch, social interactions, decision-making, learning, memory, fear, movement, perception, emotions, etc.
And more……

And more……

Cardiovascular system, oncology, ophthalmology, immunology, endocrinology and metabolism, nephrology, pharmacology, dermatology, reproductive biology, hematology, developmental biology, dentistry, gastroenterology, hepatology, respiratory system, orthopedics