Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging
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06.21/
2023
Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging
Weijian Zong, Runlong Wu, Shiyuan Chen, Junjie Wu, Hanbin Wang, Zhe Zhao, Guoqing Chen, Rui Tu, Danlei Wu, Yanhui Hu, Yangyang Xu, Yao Wang, Zhuoli Duan, Haitao Wu, Yunfeng Zhang, Jue Zhang, Aimin Wang, Liangyi Chen, andHeping Cheng
Publication: Nature Methods
Read more: Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging | Nature Methods
Abstract
We have developed a miniature two-photon microscope equipped with an axial scanning mechanism and a longworking-distance miniature objective to enable multi-plane imaging over a volume of 420 × 420 × 180 μm3 at a lateral resolution of ~1 μm. Together with the detachable design that permits long-term recurring imaging, our miniature two-photon microscope can help decipher neuronal mechanisms in freely behaving animals.
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